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SRX13867583: GSM5830726: 15C_WT_2; Xylella fastidiosa subsp. fastidiosa; RNA-Seq
1 OXFORD_NANOPORE (MinION) run: 93,497 spots, 65.8M bases, 57Mb downloads

Submitted by: NCBI (GEO)
Study: Using next generation sequencing to compare gene expression of wild type and ?csp1 mutant of bacterial plant pathogen Xylella fastidiosa strain Stag's Leap
PRJNA765323 • SRP338213 • All experiments • All runs
show Abstracthide Abstract
To investigate the role(s) of a cold shock protein homolog (Csp1) in plant pathogenic bacteria Xylella fastidiosa, we compared transcriptome profiles between wild type and a csp1 deletion mutant (?csp1) using long read Nanopore RNA sequencing. Overall design: Examined mRNA profiles of 7 day old wild type and ?csp1 X. fastidiosa cells grown on PD3 agar plates under standard conditions (28°C) and at low temperature conditions (15°C)
Sample: 15C_WT_2
SAMN25182177 • SRS11741257 • All experiments • All runs
Organism: Xylella fastidiosa subsp. fastidiosa
Library:
Instrument: MinION
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Bacteria cells were scraped off PD3 agar plates and flash frozen on dry ice. RNA was purified using Trizol reagent. Oxford Nanopore direct cDNA sequencing kit was was used with 250ng of rRNA depleted RNA for the construction of sequencing libraries. Nanopre direct cDNA sequencing library
Experiment attributes:
GEO Accession: GSM5830726
Links:
Runs: 1 run, 93,497 spots, 65.8M bases, 57Mb
Run# of Spots# of BasesSizePublished
SRR1770419793,49765.8M57Mb2022-02-25

ID:
19324727

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